Biology

Webquest Oct 28th, 2010

Students, here are the pages you need to work on.

ENZYME WEBQUEST

WEBSITE #1: http://www.northland.cc.mn.us/biology/Biology1111/animations/enzyme.html

Choose ENZYMES: The BASICS

1. SLIDE ONE: Enzymes are ________________that serve as __________________.

They _________________up or _______________ ______________reactions, but ALWAYS remain _________________.

2. SLIDE TWO: What is an ACTIVE SITE?

3. SLIDE THREE: Each ______________acts on a specific_____________.

4. What is a SUBSTRATE?

5. DRAW an ENZYME and SUBSTRATE below. Make each a DIFFERENT COLOR and label each.

6. SLIDE FIVE: YOU JUST DRANK A GLASS OF MILK! DRAW THE CATALYTIC CYCLE OF LACTOSE BELOW! LABELING THE SUBSTRATE, SUBSTRATE ENZYME COMPLEX, THE ACTIVE SITE, AND THE PRODUCTS. MAKING EACH A DIFFERENT COLOR!

7. Someone who is ?LACTOSE INTOLERANT??is lacking which enzyme?____________________

WEBSITE #2: http://www.phschool.com/science/biology_place/labbench/lab2/intro.html

Enzyme Catalysis

Key Concepts

8. _________________ catalyze reactions by lowering the _______________ _________________ necessary for a reaction to occur. The molecule that an enzyme acts on is called the _________________. In an enzyme-mediated reaction, _________________ molecules are changed, and _________________ is formed. The _________________molecule is _________________ after the reaction, and it can continue___________________________________________________________________________________________________________________________________.

9. Each _________________ is _________________for the _________________ it will catalyze. In this laboratory,

Enzyme = catalase, found in your liver

Substrate = hydrogen peroxide (H₂O₂)

Products = water and oxygen

10. DRAW THE REACTION THAT TOOK PLACE ON THE LEFT USING YOUR OWN SHAPES, LABELING THE SUBSTRATE, ENZYME, ACTIVE SITE ON THE ENZYME, ENZYME-SUBSTRATE COMPLEX, AND THE PRODUCTS.

Enzyme Structure

11. Enzymes are globular, functional _____________________. Their folded conformation creates an area known as the _________________ _______________. The nature and arrangement of __________________ ____________________ in the __________________ __________________ make it specific__________________________________________________________.

12. DRAW A MODEL OF AN ENZYME, ACTIVE SITE, AND SUBSTRATE BELOW:

Some Factors That Affect Enzyme Action

13. The conformation of an enzyme is maintained by interactions between the various ___________________ _______________ that compose it, and this conformation is sensitive to ________________________________________. Two important influences are _________________ and ________________. When an enzyme's ________________________ is significantly altered because of ____________ or ___________________ variation, the enzyme may no longer _____________________ ___________________. An enzyme is said to be _____________________ when it loses its functional shape.

pH and Enzyme Function

14. Each enzyme functions best within a _______________________. For example, the enzyme__________________, which works in your stomach, functions best in a strongly ____________________environment. Lipase, an enzyme found in your _____________________________, works best in a __________________ environment.

15. When the pH changes, the active site ____________________________ and affects ____________________ ________________. What happens to catalysis when an enzyme is subjected to a pH far from its optimum range?

16. WATCH THE ANIMATION AND DESCRIBE WHAT YOU SEE BELOW:

Temperature and Enzyme Function

17. Chemical reactions speed up as ____________________ is increased, so, in general,_________________ will ___________________ at higher temperatures. However, each enzyme has a temperature __________________________, and beyond this point the enzyme's _____________________________ is lost. ___________________ temperatures will ______________________ most enzymes.

WEBSITE#3

:http://bcs.whfreeman.com/thelifewire/content/chp06/0602001.html

GO TO ANIMATION, then to STEP THROUGH

1^st-Add a SUBSTRATE

2^nd-Add an ENZYME

PRESS PLAY

18. Describe what happened:

WEBSITE #4: http://bcs.whfreeman.com/thelifewire/content/chp06/0602002.html

Make sure you are in the ANIMATION portion and go to STEP THROUGH?Watch the animation?it is a bit complicated. Main idea I need you to understand is?.

19. TRUE or FALSE: an enzyme can change shape and become ACTIVE or INACTIVE.

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- #18. Enzymes - Active Site, Activation Energy, Enzyme Specificity
Enzymes are globular proteins that serve as biological catalysts. They speed up or slow down metabolic reaction, but remain unchanged. They may facilitate the breaking of an existing bond or the formation of a new bond....

- #17.1 Enzymes - Syllabus 2015
? Mode of action of enzymes ? Factors that affect enzyme action Learning Outcomes Candidates should be able to: (a) explain that enzymes are globular proteins that catalyse metabolic reactions; (b) explain the mode of action of enzymes in terms of...

- Enzymes
ENZYMES Enzymes are biological catalysts which influence biochemical reactions. All enzymes are proteins but all proteins are not enzymes because there are proteins other than enzymes. Ribozymes: These are the Nucleic acids (RNA) that behave like...

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