Biology
Primers mark out a sequence of DNA to amplify. This sequence would be complementary to the target DNA strand which it attaches itself to. They also prevent the single stranded DNA from re-annealling by sticking to a part of the target DNA. They also allow Taq polymerase to work from a 5' to 3' direction, since the enzyme can only work with a free 3' end.
- Q: Describe Semi-conservative Replication
A segment of DNA is unwound by helicase. H-bonds between both strands are broken. Primers are laid down by primase in the 5' to 3' direction. DNA polymerase III adds complementary free nucleotides to 3' growing end....
- Polymerase Chain Reaction (pcr)
There are three stages to PCR: denaturation, heating, and extension. In the first stage, denaturation, the reaction tube in the thermal cycler is heated to 95 degrees Celsius. The high heat denatures DNA by breaking the hydrogen bonds; the double helix...
- Q: Explain Why The Two Strands Of Dna Elongate In Opposite Directions
The two strands of DNA are anti parallel. Their 5' to 3' direction is in opposite direction. As the active site of the enzyme DNA polymerase III only recognizes the 3' end of a DNA nucleotide, free nucleotides can only be added in the 3'...
- Transcription
Each gene contains a specific nucleotide sequence called the promoter region and it contains the initiation site, where proteins, called transcription factors, and RNA polymerase enzyme recognize and bind to. The enzyme-DNA-protein complex then causes...
- Mrna Is Produced By Transcription And Is Translated
KEY TERMS:Transcription describes synthesis of RNA on a DNA template. Translation is synthesis of protein on the mRNA template. A coding region is a part of the gene that represents a protein sequence. The antisense strand (Template strand) of DNA...
Biology
Q: Explain the functions of primers in PCR
Primers mark out a sequence of DNA to amplify. This sequence would be complementary to the target DNA strand which it attaches itself to. They also prevent the single stranded DNA from re-annealling by sticking to a part of the target DNA. They also allow Taq polymerase to work from a 5' to 3' direction, since the enzyme can only work with a free 3' end.
- Q: Describe Semi-conservative Replication
A segment of DNA is unwound by helicase. H-bonds between both strands are broken. Primers are laid down by primase in the 5' to 3' direction. DNA polymerase III adds complementary free nucleotides to 3' growing end....
- Polymerase Chain Reaction (pcr)
There are three stages to PCR: denaturation, heating, and extension. In the first stage, denaturation, the reaction tube in the thermal cycler is heated to 95 degrees Celsius. The high heat denatures DNA by breaking the hydrogen bonds; the double helix...
- Q: Explain Why The Two Strands Of Dna Elongate In Opposite Directions
The two strands of DNA are anti parallel. Their 5' to 3' direction is in opposite direction. As the active site of the enzyme DNA polymerase III only recognizes the 3' end of a DNA nucleotide, free nucleotides can only be added in the 3'...
- Transcription
Each gene contains a specific nucleotide sequence called the promoter region and it contains the initiation site, where proteins, called transcription factors, and RNA polymerase enzyme recognize and bind to. The enzyme-DNA-protein complex then causes...
- Mrna Is Produced By Transcription And Is Translated
KEY TERMS:Transcription describes synthesis of RNA on a DNA template. Translation is synthesis of protein on the mRNA template. A coding region is a part of the gene that represents a protein sequence. The antisense strand (Template strand) of DNA...