Biology
It is crucial that the genetic material is reproduced accurately. Because the two polynucleotide strands are joined only by hydrogen bonds, they are able to separate without requiring breakage of covalent bonds. The specificity of base pairing suggests that each of the separated parental strands could act as a template strand for the synthesis of a complementary daughter strand. Figure 1.11 shows the principle that a new daughter strand is assembled on each parental strand. The sequence of the daughter strand is dictated by the parental strand; an A in the parental strand causes a T to be placed in the daughter strand, a parental G directs incorporation of a daughter C, and so on.
The consequences of this mode of replication are illustrated in Figure 1.12. The parental duplex is replicated to form two daughter duplexes, each of which consists of one parental strand and one (newly synthesized) daughter strand. The unit conserved from one generation to the next is one of the two individual strands comprising the parental duplex. This behavior is called semiconservative replication.
- Dna Replication
DNA Replication?DNA can copy its self over and over again in order to keep creating for the organism it is working for. This action is called DNA replication and takes place in the cell nucleus. How does DNA replication work? The molecules of DNA...
- Mrna Is Produced By Transcription And Is Translated
KEY TERMS:Transcription describes synthesis of RNA on a DNA template. Translation is synthesis of protein on the mRNA template. A coding region is a part of the gene that represents a protein sequence. The antisense strand (Template strand) of DNA...
- Arthropod Satellites Have Very Short Identical Repeats
KEY TERMS:Heavy strands and light strands of a DNA duplex refer to the density differences that result when there is an asymmetry between base representation in the two strands such that one strand is rich in T and G bases and the other is rich in C and...
- Recombination Occurs By Physical Exchange Of Dna
KEY TERMS:Crossing-over describes the reciprocal exchange of material between chromosomes that occurs during prophase I of meiosis and is responsible for genetic recombination. A bivalent is the structure containing all four chromatids (two representing...
- # 34 Dna Structurer And Replication
DNA molecule consists of nucleotides in which the sugar component is deoxyribose whereas the RNA molecule has nucleotides in which the sugar is a ribose. Nucleotides Roles: are monomers for nucleic acid polymers, such as DNA and RNA....
Biology
DNA replication is semiconservative
KEY TERMS:
- A parental strand or duplex of DNA refers to the DNA that will be replicated.
- The antisense strand (Template strand) of DNA is complementary to the sense strand, and is the one that acts as the template for synthesis of mRNA.
- A daughter strand or duplex of DNA refers to the newly synthesized DNA.
- Semiconservative replication is accomplished by separation of the strands of a parental duplex, each then acting as a template for synthesis of a complementary strand.
KEY CONCEPTS:
- The Meselson-Stahl experiment used density labeling to prove that the single polynucleotide strand is the unit of DNA that is conserved during replication.
- Each strand of a DNA duplex acts as a template to synthesize a daughter strand.
- The sequences of the daughter strands are determined by complementary base pairing with the separated parental strands.
It is crucial that the genetic material is reproduced accurately. Because the two polynucleotide strands are joined only by hydrogen bonds, they are able to separate without requiring breakage of covalent bonds. The specificity of base pairing suggests that each of the separated parental strands could act as a template strand for the synthesis of a complementary daughter strand. Figure 1.11 shows the principle that a new daughter strand is assembled on each parental strand. The sequence of the daughter strand is dictated by the parental strand; an A in the parental strand causes a T to be placed in the daughter strand, a parental G directs incorporation of a daughter C, and so on.The top part of the figure shows a parental (unreplicated) duplex that consists of the original two parental strands. The lower part shows the two daughter duplexes that are being produced by complementary base pairing. Each of the daughter duplexes is identical in sequence with the original parent, and contains one parental strand and one newly synthesized strand. The structure of DNA carries the information needed to perpetuate its sequence.
The consequences of this mode of replication are illustrated in Figure 1.12. The parental duplex is replicated to form two daughter duplexes, each of which consists of one parental strand and one (newly synthesized) daughter strand. The unit conserved from one generation to the next is one of the two individual strands comprising the parental duplex. This behavior is called semiconservative replication.The figure illustrates a prediction of this model. If the parental DNA carries a "heavy" density label because the organism has been grown in medium containing a suitable isotope (such as 15N), its strands can be distinguished from those that are synthesized when the organism is transferred to a medium containing normal "light" isotopes.
The parental DNA consists of a duplex of two heavy strands (red). After one generation of growth in light medium, the duplex DNA is "hybrid" in density?it consists of one heavy parental strand (red) and one light daughter strand (blue). After a second generation, the two strands of each hybrid duplex have separated; each gains a light partner, so that now half of the duplex DNA remains hybrid while half is entirely light (both strands are blue).
The individual strands of these duplexes are entirely heavy or entirely light. This pattern was confirmed experimentally in the Meselson-Stahl experiment of 1958, which followed the semiconservative replication of DNA through three generations of growth of E. coli. When DNA was extracted from bacteria and its density measured by centrifugation, the DNA formed bands corresponding to its density?heavy for parental, hybrid for the first generation, and half hybrid and half light in the second generation (Meselson and Stahl, 1958; for review see Holmes, 2001).
- Dna Replication
DNA Replication?DNA can copy its self over and over again in order to keep creating for the organism it is working for. This action is called DNA replication and takes place in the cell nucleus. How does DNA replication work? The molecules of DNA...
- Mrna Is Produced By Transcription And Is Translated
KEY TERMS:Transcription describes synthesis of RNA on a DNA template. Translation is synthesis of protein on the mRNA template. A coding region is a part of the gene that represents a protein sequence. The antisense strand (Template strand) of DNA...
- Arthropod Satellites Have Very Short Identical Repeats
KEY TERMS:Heavy strands and light strands of a DNA duplex refer to the density differences that result when there is an asymmetry between base representation in the two strands such that one strand is rich in T and G bases and the other is rich in C and...
- Recombination Occurs By Physical Exchange Of Dna
KEY TERMS:Crossing-over describes the reciprocal exchange of material between chromosomes that occurs during prophase I of meiosis and is responsible for genetic recombination. A bivalent is the structure containing all four chromatids (two representing...
- # 34 Dna Structurer And Replication
DNA molecule consists of nucleotides in which the sugar component is deoxyribose whereas the RNA molecule has nucleotides in which the sugar is a ribose. Nucleotides Roles: are monomers for nucleic acid polymers, such as DNA and RNA....