Biology
Advantages:
PCR is highly sensitive as it is capable of amplifying sequences from minute sequences of target DNA. It also has high fidelity as it produces the sequences required with a low error rate. It is also rapid as it can generate many copies within a short period of time. It is a quick and reliable method for detecting all mutations. Moreover, amplification can be done from unusual sources e.g. Egyptian mummies where the DNA quality is not good due to degradation.
Limitations:
Some knowledge of DNA/amino acid sequence of desired gene/protein is needed for the synthesis of flanking primers.
Non-target DNA sequence may be amplified instead, as primers are short and there is the possibility of many sequences complementary to primers. Also, Taq polymerase does not perform proofreading so there is the possibility of errors
- Polymerase Chain Reaction (pcr)
There are three stages to PCR: denaturation, heating, and extension. In the first stage, denaturation, the reaction tube in the thermal cycler is heated to 95 degrees Celsius. The high heat denatures DNA by breaking the hydrogen bonds; the double helix...
- Mrna Splicing
Genes of eukaryotic cells containing introns and exons. Introns, short for intervening sequences, are non-coding regions of a gene. They are transcribed but not translated into the amino acid sequence of proteins. Exons, or expressed sequences, are coding...
- Arthropod Satellites Have Very Short Identical Repeats
KEY TERMS:Heavy strands and light strands of a DNA duplex refer to the density differences that result when there is an asymmetry between base representation in the two strands such that one strand is rich in T and G bases and the other is rich in C and...
- Exon Sequences Are Conserved But Introns Vary
KEY CONCEPTS:Comparisons of related genes in different species show that the sequences of the corresponding exons are usually conserved but the sequences of the introns are much less well related. Introns evolve much more rapidly than exons because of...
- Restriction Endonucleases Are A Key Tool In Mapping Dna
KEY TERMS:Restriction endonucleases recognize specific short sequences of DNA and cleave the duplex (sometimes at target site, sometimes elsewhere, depending on type). A restriction map is a linear array of sites on DNA cleaved by various restriction...
Biology
Q: List the advantages and limitations of PCR
Advantages:
PCR is highly sensitive as it is capable of amplifying sequences from minute sequences of target DNA. It also has high fidelity as it produces the sequences required with a low error rate. It is also rapid as it can generate many copies within a short period of time. It is a quick and reliable method for detecting all mutations. Moreover, amplification can be done from unusual sources e.g. Egyptian mummies where the DNA quality is not good due to degradation.
Limitations:
Some knowledge of DNA/amino acid sequence of desired gene/protein is needed for the synthesis of flanking primers.
Non-target DNA sequence may be amplified instead, as primers are short and there is the possibility of many sequences complementary to primers. Also, Taq polymerase does not perform proofreading so there is the possibility of errors
- Polymerase Chain Reaction (pcr)
There are three stages to PCR: denaturation, heating, and extension. In the first stage, denaturation, the reaction tube in the thermal cycler is heated to 95 degrees Celsius. The high heat denatures DNA by breaking the hydrogen bonds; the double helix...
- Mrna Splicing
Genes of eukaryotic cells containing introns and exons. Introns, short for intervening sequences, are non-coding regions of a gene. They are transcribed but not translated into the amino acid sequence of proteins. Exons, or expressed sequences, are coding...
- Arthropod Satellites Have Very Short Identical Repeats
KEY TERMS:Heavy strands and light strands of a DNA duplex refer to the density differences that result when there is an asymmetry between base representation in the two strands such that one strand is rich in T and G bases and the other is rich in C and...
- Exon Sequences Are Conserved But Introns Vary
KEY CONCEPTS:Comparisons of related genes in different species show that the sequences of the corresponding exons are usually conserved but the sequences of the introns are much less well related. Introns evolve much more rapidly than exons because of...
- Restriction Endonucleases Are A Key Tool In Mapping Dna
KEY TERMS:Restriction endonucleases recognize specific short sequences of DNA and cleave the duplex (sometimes at target site, sometimes elsewhere, depending on type). A restriction map is a linear array of sites on DNA cleaved by various restriction...