| Vertebrates | Invertebrates |
| 1. Notochord is present in the embryo and vertebral column in the adult | Notochord and vertebral column are absent in embryo and adult |
| 2. Gill slits are present | Absent |
| 3. Nerve cord is single, hollow and non-ganglionated | Nerve cord is double, solid and ganglionated |
| 4. Cranium is present | Absent |
| 5. Post-anal tail is present | Absent |
| 6. Two pairs of appendages for locomotion | Many pairs when present |
| 7. Heart is ventral to the alimentary canal | Heart is dorsal to the alimentary canal |
| 8. Blood flows forward ventrally and backward dorsally | Blood flows forward dorsally and backward ventrally |
| 9. Haemoglobin is present in RBC | Haemoglobin if present is dissolved in plasma |
| 10. Endoskeleton is present | Absent |