| Gram positive bacteria | Gram negative bacteria | 
| 1.       The bacteria remain coloured with   Gram staining even after washing with alcohol or acetone | The bacteria do not   retain the stain when washed with alcohol or acetone. So these type of bacteria   are observed with another stain such as safranin.  | 
| 2.       Outer membrane is absent | Present | 
| 3.       Thicker cell wall. Thickness   varies from 20-80 nm | Thin cell wall.   Thickness varies from 8-12 nm | 
| 4.       Smooth wall | Wavy wall and comes   in contact with plasma membrane only at a few loci. | 
| 5.       The wall contains 70-80% peptidoglycan   (murein or mucopeptide) | The wall contains 10-20%   peptidoglycan  | 
| 6.       Teichoic acids may be present | Teichoic acids   absent | 
| 7.       Very little lipid content (0-2%) | High lipid content   (20-30%) | 
| 8.       Lipopolysaccharide layer (LPS)   absent. | Present  | 
| 9.       Basal body of the flagellum   contains 2 rings | Basal body of the   flagellum contains 4 rings | 
| 10.    Mesosomes are quite prominent | Mesosomes are less   prominent | 
| 11.    A few pathogenic bacteria belong   to Gram positive group | Most of the   pathogenic bacteria belong to Gram negative group | 
| 12.    E.g. Bacillus subtilis, Mycobacterium,   Noccardia | E.g. Escherichia   coli |