Gram positive bacteria | Gram negative bacteria |
1. The bacteria remain coloured with Gram staining even after washing with alcohol or acetone | The bacteria do not retain the stain when washed with alcohol or acetone. So these type of bacteria are observed with another stain such as safranin. |
2. Outer membrane is absent | Present |
3. Thicker cell wall. Thickness varies from 20-80 nm | Thin cell wall. Thickness varies from 8-12 nm |
4. Smooth wall | Wavy wall and comes in contact with plasma membrane only at a few loci. |
5. The wall contains 70-80% peptidoglycan (murein or mucopeptide) | The wall contains 10-20% peptidoglycan |
6. Teichoic acids may be present | Teichoic acids absent |
7. Very little lipid content (0-2%) | High lipid content (20-30%) |
8. Lipopolysaccharide layer (LPS) absent. | Present |
9. Basal body of the flagellum contains 2 rings | Basal body of the flagellum contains 4 rings |
10. Mesosomes are quite prominent | Mesosomes are less prominent |
11. A few pathogenic bacteria belong to Gram positive group | Most of the pathogenic bacteria belong to Gram negative group |
12. E.g. Bacillus subtilis, Mycobacterium, Noccardia | E.g. Escherichia coli |