| Enzymes | Hormones |
| 1. All enzymes are proteins | They may be proteins, amines or steroids |
| 2. They are macromolecules with higher molecular weight | They have only low molecular weight |
| 3. They are non-diffusible through cell membrane | They are diffusible through cell membrane |
| 4. They either act intracellularly or carried by some ducts to another site | Generally carried by blood to a target organ |
| 5. Always act as biological catalysts and increases the rate of metabolic physiological processes. | They may be either excitatory or inhibitory in their action |
| 6. They catalyze reversible reactions | Hormone controlled reactions are not reversible |
| 7. Reaction rate increases with increase in their concentration up to a limit. | Deficiency or overproduction of hormone causes metabolic disorders or diseases. |
| 8. They act quickly | Some hormones are quick acting, while some are slow acting with a lag period. |
| 9. They are not used in metabolic functions | They are used up in metabolic functions. |