Biology
- Q: Explain How A Foreign Dna Fragment Can Be Introduced Into A Plasmid That Produces Blunt Ends.
Cut the DNA fragment and plasmid using the same restriction enzyme. Add guanines to DNA fragment blunt ends and terminal transferase, to produce a single chain of guanines to both the blunt ends of the plasmid vector. Add cytosines to vector blunt ends,...
- Bacterial Mrna Degradation Involves Multiple Enzymes
KEY TERMS:The degradosome is a complex of bacterial enzymes, including RNAase and helicase activities, which may be involved in degrading mRNA. KEY CONCEPTS: The overall direction of degradation of bacterial mRNA is 5?3. Degradation results from the...
- Individual Genomes Show Extensive Variation
KEY TERMS:Polymorphism (more fully genetic polymorphism) refers to the simultaneous occurrence in the population of genomes showing variations at a given position. The original definition applied to alleles producing different phenotypes. Now it is also...
- Genomes Can Be Mapped By Linkage, Restriction Cleavage, Or Dna Sequence
Defining the contents of a genome essentially means making a map. We can think about mapping genes and genomes at several levels of resolution:A genetic (or linkage) map identifies the distance between mutations in terms of recombination frequencies....
- Gel Electrophoresis Is Used For
A) Isolation of DNA molecule B) Separation of DNA fragments according to size C) Cutting DNA into fragments D) Construction of recombinant DNA The correct answer is: B) Separation of DNA fragments according to size...
Biology
Which of these enzymes is used to join cut fragments of DNA?
A) Restriction Exonuclease
B) Restriction Endonuclease
C) DNA Polymerase
D) DNA Ligase
The correct answer is:
D) DNA Ligase
B) Restriction Endonuclease
C) DNA Polymerase
D) DNA Ligase
The correct answer is:
D) DNA Ligase
- Q: Explain How A Foreign Dna Fragment Can Be Introduced Into A Plasmid That Produces Blunt Ends.
Cut the DNA fragment and plasmid using the same restriction enzyme. Add guanines to DNA fragment blunt ends and terminal transferase, to produce a single chain of guanines to both the blunt ends of the plasmid vector. Add cytosines to vector blunt ends,...
- Bacterial Mrna Degradation Involves Multiple Enzymes
KEY TERMS:The degradosome is a complex of bacterial enzymes, including RNAase and helicase activities, which may be involved in degrading mRNA. KEY CONCEPTS: The overall direction of degradation of bacterial mRNA is 5?3. Degradation results from the...
- Individual Genomes Show Extensive Variation
KEY TERMS:Polymorphism (more fully genetic polymorphism) refers to the simultaneous occurrence in the population of genomes showing variations at a given position. The original definition applied to alleles producing different phenotypes. Now it is also...
- Genomes Can Be Mapped By Linkage, Restriction Cleavage, Or Dna Sequence
Defining the contents of a genome essentially means making a map. We can think about mapping genes and genomes at several levels of resolution:A genetic (or linkage) map identifies the distance between mutations in terms of recombination frequencies....
- Gel Electrophoresis Is Used For
A) Isolation of DNA molecule B) Separation of DNA fragments according to size C) Cutting DNA into fragments D) Construction of recombinant DNA The correct answer is: B) Separation of DNA fragments according to size...